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Bio20 Problem Set 2 (1718-2)

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  30    ore BIO20 Introduction to Biomimetics Engg. and Component Design  PROBLEM SET 2: CENTRAL DOGMA OF MOLECULAR GENETICS and RECOMBINANT DNA TECHNOLOGY  Name/s:__________________________ Signature/s:_________________ Date submitted: __________________________ _________________ Section: __________________________ _________________  __________________________ _________________ Direction: Answer completely the following questions. I. Gien the DNA sequence: 5’ –ATG CAT CGC GAC ATA CAG ACC AAA TTT TGA … 3’  a. Gie its complementary DNA sequence. b. Gie the product when it undergoes transcription. c. Gie the product of translation of the transcribed sequence. II. Gien the Normal DNA Sequence: 5’ –ATG CAT CGC GAC ATA CAG ACC AAA TTT TGA … 3’  Determine what type of mutation occurred in the following mutant DNA.!utant : 5’ –ATG CAT CGC GAC ATA CAG AGC AAA TTT TGA … 3’  a.At what codon number did mutation occurred# ___________________ b. dentify the type of substitution point mutation happened. ____________________________ c.$hat is the resulting amino acid of the codon that was mutated# d.$hat is the consequence of that mutation#!utant : 5’ –ATG CAT CGC GAC ATA TAG ACC AAA TTT TGA … 3’ a.  At what codon number did mutation occurred# ___________________  b. dentify the type of substitution point mutation happened. _____________________________  c. $hat is the resulting amino acid of the codon that was mutated# d. $hat is the consequence of that mutation#  III. Direction: Answer completely the following questions. 1.Enumerate the general steps inRecombinant DNA echnolog!. #pts$2.%hat is pol!merase chain reaction &CR$'Enumerate the general steps in &CR. (pts$).Describe the *ollo+ing techni,ues and +hatdo the! *unction *or (pts$a$ -el Electrophoresis b$ outhern Blot /!bridiation Ans+er utaseilla%mapua.edu.ph  INTRODUCTION TO BIOMIMETICS ENGINEERING  AND COMPONENT DESIGN &     (. DNA is isolated *rom a hair sample taen*rom a comb dropped at the scene o* a banrobber!. A DNA *ingerprint is deri3ed *rom thesample labelled /$ and *rom 4 suspects numbered$. 5sing the results *rom a multi6locusprobe sho+n belo+7 +hat conclusions +ould!ou lie to mae' o +hom is the hair mostliel! to belong' (pts$
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