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Isolation of mitochondria from plant cell culture

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Summary Mitochondria carry out a variety of important processes in plants. Their major role is the synthesis of ATP through the coupling of a membrane potential to the transfer of electrons from NADH to O2 via the electron transport chain. The NADH
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  2D PAGE: Sample Preparation and Fractionation  M E T H O D S I N M O L E C U L A R B I O L O G Y TM  John M. Walker ,  S  ERIES   E   DITOR 460. Essential Concepts in Toxicogenomics,  edited by  Donna L. Mendrick and William B. Mattes, 2008 459. Prion Protein Protocols,  edited by  Andrew F. Hill,2008 458. Artificial Neural Networks:  Methods and  Applications,  edited by  David S. Livingstone, 2008 457. Membrane Trafficking,  edited by  Ales Vancura,2008 456. Adipose Tissue Protocols,  Second Edition,  edited by Kaiping Yang, 2008 455. Osteoporosis  ,  edited by  Jennifer J. Westendorf, 2008 454. SARS- and Other Coronaviruses:  LaboratoryProtocols,  edited by  Dave Cavanagh, 2008 453. Bioinformatics, Volume 2:  Structure, Function, and  Applications,  edited by  Jonathan M. Keith, 2008 452. Bioinformatics, Volume 1:  Data, Sequence Analysis,and Evolution,  edited by  Jonathan M. Keith, 2008 451. Plant Virology Protocols:  From Viral Sequence toProtein Function,  edited by  Gary Foster, Elisabeth Johansen, Yiguo Hong, and Peter Nagy, 2008 450. Germline Stem Cells,  edited by  Steven X. Hou and Shree Ram Singh, 2008 449. Mesenchymal Stem Cells:  Methods and Protocols, edited by  Darwin J. Prockop, Douglas G. Phinney,and Bruce A. Brunnell, 2008 448. Pharmacogenomics in Drug Discovery andDevelopment,  edited by  Qing Yan, 2008 447. Alcohol:  Methods and Protocols,  edited by  Laura E. Nagy, 2008 446. Post-translational Modification of Proteins:  Tools for Functional Proteomics, Second Edition,  edited by Christoph Kannicht, 2008 445. Autophagosome and Phagosome,  edited by Vojo Deretic, 2008 444. Prenatal Diagnosis,  edited by  Sinhue Hahn and  Laird G. Jackson, 2008 443. Molecular Modeling of Proteins,  edited by  Andreas Kukol, 2008 442. RNAi:  Design and Application,  edited by  Sailen Barik, 2008 441. Tissue Proteomics:  Pathways, Biomarkers, and Drug Discovery,  edited by  Brian Liu, 2008 440. Exocytosis and Endocytosis,  edited by  Andrei I. Ivanov, 2008 439. Genomics Protocols,  Second Edition,  edited by  Mike Starkey and Ramnanth Elaswarapu, 2008 438. Neural Stem Cells:  Methods and Protocols, Second Edition,  edited by  Leslie P. Weiner, 2008 437. Drug Delivery Systems,  edited by  Kewal K. Jain,2008 436. Avian Influenza Virus,  edited by  Erica Spackman,2008 435. Chromosomal Mutagenesis,  edited by  Greg Davisand Kevin J. Kayser, 2008 434. Gene Therapy Protocols:  Volume 2, Design and Characterization of Gene Transfer Vectors,  edited by  Joseph M. LeDoux, 2008 433. Gene Therapy Protocols:  Volume 1, Production and  In Vivo Applications of Gene Transfer Vectors,  editedby  Joseph M. LeDoux, 2008 432. Organelle Proteomics,  edited by  Delphine Pflieger and Jean Rossier, 2008 431. Bacterial Pathogenesis:  Methods and Protocols, edited by  Frank DeLeo and Michael Otto, 2008 430. Hematopoietic Stem Cell Protocols,  edited by  Kevin D. Bunting, 2008 429. Molecular Beacons:  Signalling Nucleic Acid Probes, Methods and Protocols,  edited by  Andreas Marx and Oliver Seitz, 2008 428. Clinical Proteomics:  Methods and Protocols,  editedby  Antonio Vlahou, 2008 427. Plant Embryogenesis,  edited by  Maria FernandaSuarez and Peter Bozhkov, 2008 426. Structural Proteomics:  High-Throughput Methods, edited by  Bostjan Kobe, Mitchell Guss, and Huber Thomas, 2008 425. 2D PAGE:  Sample Preparation and Fractionation,Volume 2,  edited by  Anton Posch, 2008 424. 2D PAGE:  Sample Preparation and Fractionation,Volume 1,  edited by  Anton Posch, 2008 423. Electroporation Protocols,  edited by  Shulin Li, 2008 422. Phylogenomics,  edited by  William J. Murphy, 2008 421. Affinity Chromatography,  Methods and Protocols,Second Edition,  edited by  Michael Zachariou, 2007  420. Drosophila,  Methods and Protocols,  edited by Christian Dahmann, 2008 419. Post-Transcriptional Gene Regulation,  edited by  Jeffrey Wilusz, 2008 418. Avidin-Biotin Interactions,  Methods and  Applications,  edited by  Robert J. McMahon, 2008 417. Tissue Engineering, Second Edition,  edited by  Hannsjörg Hauser and Martin Fussenegger, 2007  416. Gene Essentiality:  Protocols and Bioinformatics, edited by  Andrei L. Osterman, 2008 415. Innate Immunity,  edited by  Jonathan Ewbank and Eric Vivier, 2007  414. Apoptosis and Cancer:  Methods and Protocols, edited by  Gil Mor and Ayesha B. Alvero, 2008 413. Protein Structure Prediction, Second Edition, edited by  Mohammed Zaki and Chris Bystroff, 2008 412. Neutrophil Methods and Protocols,  edited by  Mark T. Quinn, Frank R. DeLeo, and Gary M. Bokoch,2007  411. Reporter Genes for Mammalian Systems,  edited by  Don Anson, 2007  410. Environmental Genomics,  edited by Cristofre C. Martin, 2007  409. Immunoinformatics:  Predicting Immunogenicity InSilico, edited by Darren R. Flower, 2007   M E T H O D S I N M O L E C U L A R B I O L O G Y TM 2D PAGE: SamplePreparationand Fractionation Volume 2 Edited by Anton Posch  Bio-Rad Laboratories GmbH, Munich, Germany  Editor  Anton PoschBio-Rad Laboratories GmbHMunich, Germany Series Editor  John M. WalkerSchool of Life SciencesUniversity of HertfordshireHatfield, Herts., UK ISBN: 978-1-60327-209-4 e-ISBN: 978-1-60327-210-0ISSN: 1064-3745Library of Congress Control Number: 2007943017©2008 Humana Press, a part of Springer Science+Business Media, LLCAll rights reserved. This work may not be translated or copied in whole or in part without the writtenpermission of the publisher (Humana Press, 999 Riverview Drive, Suite 208, Totowa, NJ 07512 USA),except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any formof information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilarmethodology now known or hereafter developed is forbidden.The use in this publication of trade names, trademarks, service marks, and similar terms, even if they arenot identified as such, is not to be taken as an expression of opinion as to whether or not they are subject toproprietary rights.While the advice and information in this book are believed to be true and accurate at the date of going topress, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errorsor omissions that may be made. The publisher makes no warranty, express or implied, with respect to thematerial contained herein. Cover illustration : Figure 3, Chapter 14, “The Terminator: A Device for High Throughput Extraction of Plant Material,” by B. M. van den Berg.Printed on acid-free paper9 8 7 6 5 4 3 2 1springer.com
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